Gst fusion protein manual

User Manual BaculoDirect™ GST Gateway® Expression Kit For cloning and high-level expression of recombinant GST-fusion proteins using Gateway®-adapted Baculovirus DNA Catalog nos. A, A Rev. Date: 14 July Manual part no. A MAN The Glutathione-S-Transferase (GST) “pulldown” assay has been used extensively to assay protein interactions in vitro. This methodology has been especially useful for investigating the interactions of nuclear hormone receptors with a. Detailed information about the production of GST-tagged proteins can be found in the GST Gene Fusion System Handbook (see Ordering information). Within the United States of America [USA] and the Territories of the USA, this product is To Be Used Solely for Research And Development Purposes Under the Direct Supervision of a.

Pierce GST Protein Interaction Pull-Down Kit, contains sufficient materials for conducting 25 pull-down assays using a GST-tagged fusion protein as the bait. Kit Contents: Glutathione Agarose, µL settled resin (mL of 50% slurry in preservative buffer); binding capacity ≥ 10mg GST-tagged protein per milliliter of settled resin. immobilized on paramagnetic particles (MagneGST™ Glutathione Particles) is used to isolate GST-fusion proteins directly from a crude cell lysate using a manual or automated procedure. Using the manual protocol, GST-fusion proteins can be purifi ed on a small scale from 1ml of culture or on a large scale from up to 50ml of culture. Although. fusion protein can be cleaved at the GST tag using thrombin, HRV 3C protease, or Thermo Scientific Factor Xa (Product No. ). Important Product Information • Protein yield and purity are dependent upon the expression level, conformation and solubility characteristics of the recombinant fusion protein.

13 de jan. de GST fusion protein aggregates interfere with reproducible Kit (iNtRON Biotechnology) according to the manufacturer's instructions. cleotide but not the protein radiolabeled with 35S-methionine. Molecular Cloning: A Laboratory Manual, length GST-RoazD86 fusion protein. Typical GST fusion proteins can be produced from 1 litre of www.doorway.ru BL21 cells grown in a rich medium. A standard induction protocol entails shifting log-.